Monitoring and robust adaptive control of fed-batch cultures of microorganisms exhibiting overflow metabolism [abstract]

Monitoring and robust adaptive control of fed-batch cultures of microorganisms exhibiting overflow metabolism [abstract]

Blog Article

Overflow metabolism characterizes cells strains that are likely to produce inhibiting by-products resulting from an excess of substrate feeding and a saturated respiratory capacity.The critical substrate level separating the two different metabolic pathways is generally not well defined.Monitoring of this kind of cultures, going from model identification Ethernet-Over-Power to state estimation, is first discussed.Then, a review of control techniques which all aim at maximizing the cell productivity of fed-batch fermentations Eyeliner is presented.

Two main adaptive control strategies, one using an estimation of the critical substrate level as set-point and another regulating the by-product concentration, are proposed.Finally, experimental investigations of an adaptive RST control scheme using the observer polynomial for the regulation of the ethanol concentration in Saccharomyces cerevisiae fed-batch cultures ranging from laboratory to industrial scales, are also presented.